RCL2®, a true solution for formalin-free fixation for a better service to Patient
 
Tissues fixation has been for decades using formaldehyde based reagents such as formalin or AFA (alcohol+formalin+acetic acid). Formaldehyde reacts with the bio specimen by creating covalent bounds between biological macromolecules, thus stabilizing them and inhibiting enzymatic activities.
While of massive use in all pathology labs of the world whether in human or animal histology, formalin and its variations demonstrate two major well known for ages disadvantages:

(1) Formalin is a highly toxic reagent. Classified carcinogenic of class I by WHO since October 2006, it is demonstrated to be at the proven beginning of respiratory pathologies, allergies and respiratory tract cancers.
 
(2) The chemical action of formalin binds severely DNA, RNA and proteins and makes them difficult or even impossible to extract in adequate quantitiesand qualities for expanding molecular predictive tests to new targeted treatments.

Developed since 1999 by Dr Philippe ROCHAIX, pathologist at the Department of Pathology of Professor VOIGT, Cancer Research Centre Claudius Regaud, Toulouse, France, RCL2® is a new, truly innovative tissue fixative, preserving tissue structures and cells.
 
Make molecular diagnosis on each and every tissue specimen for a better service to the Patient thanks to RCL2®

Specimen formalin fixation is a major obstacle to medical research and the development of molecular techniques for patient profiling in the identification of targeted therapies. RCL2® fixes tissues while preserving histological and molecular components at a high level of quality and yield.
By changing to RCL2®, you facilitate Patient access to most modern and efficient therapeutic methods to better cure without altering unnecessarily Patient quality of life and while preserving Health costs.
Adopt RCL2® and move your lab to the molecular pathology era. Change for a better service to Patient.
ALPHELYS will bring you all the required technical support in protocols optimization to speed up the transition process.

RCL2® allows you to combine histology and molecular techniques on the exact same specimen for a higher efficiency in your Research

The use of formalin in tissue fixation for diagnosis and histology obliges laboratories to deep-freeze part of each specimen for molecular research applications (read below). Thus, molecular biology techniques are achieved on frozen specimen different from the fixed tissues which were used to obtain the lesion type and the diagnosis.
Most of tissue and frozen tumour banks do not have the required massive resources to do a systematic quality control on each frozen tissue and guarantee that it corresponds to the correct lesion type.
Using RCL2® allows the researcher to work exactly on the same exact tissue to used for diagnosis dramatically improving efficiency and accuracy of your Research projects. RCL2® is the only fixative that produces specimens of quality compatible with molecular techniques even after room temperature traditional fixation, without changing your procedures

RCL2®, the only truly non toxic tissue fixative for Health and Environment

RCL2® components, which preparation is patent protected, are demonstrated without risks for Human health and Environment since decades in compliance with European or American regulations and are even authorized for Human food.
RCL2® fixative has thus absolutely no risk to be one day in the future classified as dangerous unlike some other formalin-free fixatives that still contain dialdehydes, molecule of similar activity to formaldehyde and that can show dangers for Human health.
Using RCL2® preserves your staff and the efficiency of your lab by eliminating any pathogenic and carcinogenic risks of formalin.
Are you ready to invest efforts to change your fixative and take the risk that it could be classified dangerous in the next future and that a new regulation obliges you to change again? Make the right choice at once, not a compromise, adopt RCL2®.

Achieve budget savings with RCL2®

You think that the new fixatives to substitute formalin are more expensive than formalin?
Make a more global assessment of your costs.
Adopting specialized bench equipment to protect your staff from formalin vapours is expensive. You have to maintain a permanent audit of your environment to prevent contamination of your staff or even worse a temporary interruption of your activity by medical inspectors.

The management of toxic wastes, their collect and treatment by specialized contractors is also costive and involve your liability.
Make your cost assessment more globally and you will realize that RCL2® allows you to save budgets while ensuring safety and simplicity of use for you, your staff and your Environment.

Purchase serenity for yourself with RCL2®.

A true alternate solution for formalin-free fixation is now available and validated.
RCL2® brings numerous benefits for Research, Healh economy, Environment and quality of service to Patient.
Contact us to try RCL2® and move your lab into the era of molecular histo-pathology.

Publications

1. DELFOUR C, ROGER P, BRET C, BERTHE ML, ROCHAIX P, KALFA N, RAYNAUD P, BIBEAU F, MAUDELONDE T, BOULLE N. RCL2, a new fixative, preserves morphology and nucleic acid integrity in paraffin-embedded breast carcinoma and microdissected breast tumor cells. J Mol Diagn. 2006;8:157-69.
2. LACROIX-TRIKI M, LAMANT L, ALLAL CUIDER B, DECHA A, VOIGT J-J, ROCHAIX P. RCL2® a new non toxic fixative allowing extraction of high quality DNA, mRNA and proteins from paraffin embedded tissues. (En cours de soumission)
 

Warm thanks to

Dr Philippe ROCHAIX, Research Cancer Institute Claudius Regaud, Toulouse, France, for its novelty in the invention of this genius product.

Research Cancer Institute Claudius Regaud and its General Management for having offered to ALPHELYS this exceptional opportunity to develop and market RCL2®.

Ms Claudia BEVILACQUA, Unit of Functional and Physiological Genomic of milk production, National Institute of Research in Agronomics INRA, Jouy-en-Josas, France, for her work on RCL2® and for having let us use her images and data.

Dr Nathalie BOULLE, National Institute for Health and Medical Research INSERM Unit U540, Hôpital Arnaud de Villeneuve, Montpellier, France, for her work on RCL2® and for having let us use her images and data.

To order
RCL2®
Product number 01-RCL2-CS100, 18 litres of concentrated solution